Eric LECLERC
CNRS Researcher since November 2003
AFFILIATION
Université de
Technologie de Compiègne
Biomechanics and Biomedical Engineering (CNRS-UMR
6600)
Bioengineering Department
Laboratory website: http://www.utc.fr/umr6600/
ADDRESS
Leclerc Eric
CNRS UMR 6600
Laboratoire de
biomécanique et génie biomédical
Université de
Technologie de Compiègne
B.P. 20529,
60205 Compiègne, France
CONTACT
Tel: 33 (0)3 44 23 79 43, email: eric.leclerc@utc.fr
Research
interests
My research is
focussed on the development of microfluidic
and cellular microdevices for in vitro biology investigation. My
interest is to build microtools as new tools and alternative methods to
characterize biomechanical/biochemical/biological interactions involved in
biological systems. In this frame, I
joined the Biomechanics and Biomedical Engineering
group of the University and develop a research area at the interface of
microtechnology, bioengineering and bioartificial organs groups.
Related publications
Baudoin R., Duval J.L., Griscom L.,
Baudoin R., Legallais C., Leclerc E., Selective control
of liver and kidney cells migration
during organotypic co-cultures inside fibronectin coated
rectangular silicone microchannels, Biomaterials,
accepted , in press
Baudoin R., Corlu A., Griscom L.,
Legallais C., Leclerc E., Trends in the development of microfluidic cell
biochips for in vitro hepatotoxicity, Toxicology
in vitro, accepted, in press
Leclerc E.,
Corlu A., Griscom L., Baudoin R., Legallais C.,Guidance of liver
and kidney organotipic cultures inside rectangular silicone microchannels, Biomaterials,
27:22, 4109-4119, 2006
Leclerc E.,
David B., Griscom L., Lepioufle B., Fujii T., Sakai Y., Legallais C., Perfusion
culture of mammalian cells in microfluidic environments for tissue engineering
applications, La houille blanche, 3,
2006
Leclerc E.,
David B., Griscom L., Lepioufle B., Fujii T., Layrolle P., Legallais C., Study of osteoblastic cells in a
microfluidic environment, Biomaterials, 27:4, 586-595, 2006
PREVIOUS POSITION
02/2001 – 10/2003 junior researcher in the
laboratory of the Pr
Teruo Fujii at the Institute of Industrial Science at the Tokyo University
in the frame of LIMMS
(Laboratory of Integrated Micro-Mechatronic Systems)
EDUCATION
10/1997 - 11/2000
PhD Student in
Fluids Mechanics and Energetics at the CEA (French Nuclear Energy Commission)
at the Thermalhydraulics and Physics Department
1996 -
1997 M.D. Student in
Paris VI University (http://www.lmm.jussieu.fr/DEA/dea.html)
1995 -
1996 Undergraduate Student in Marseille II
University
PREVIOUS RESEARCH PROJECTS
2003 Research Project (30 months):
Abstract
We fabricated various types of bioreactors composed of microstructured
PolyDiMethylSiloxane (PDMS) or biodegradable (pCLLA acrylate) polymers layers
for perfusion culture of mammalian cells such as hepatocytes. During the perfusion culture of the bioreactors
for one week to two weeks, cells showed good attachment, spreading and reached
their confluence over the channels of the bioreactors. In addition, the albumin production of the
hepatocytes was significantly enhanced in
the perfusion culture using the PDMS bioreactors when compared in dish-level
static culture. Although, the cellular activities of the cells
are still low even compared to in vivo tissue, the conclusions of this work is
encouraging toward future liver tissue engineering based on in vitro
propagation and maturation of hepatocyte combined with microfabrication
technologies.
Keywords: PDMS micro fabrication, bioreactors, tissue cell
cultures, perfusion
Contact : eric.leclerc@utc.fr
Contact : tfujii@iis.u-tokyo.ac.jp
Related websites:
Linked publications:
Patends:
Fujii T, Sakai Y , Leclerc E, brevet japonais (en japonais,
« cell culture chamber ») no 2002-321859
Fujii T, Sakai Y , Leclerc E, brevet japonais (en japonais,
« cell culture device, bioreactor and cell culture chamber ») no 2002-316136
Papers in journals
Leclerc E. Sakai Y., Fujii T., Cells culture in a three-dimensional network
of PDMS (PolyDiMethylSiloxane) microchannels, biomedical microdevices,
5:2, 109-114, 2003
Leclerc
E., Sakai Y. and Fujii T., A microfluidic PDMS (polydimethylsiloxane)
bioreactor for large-scale culture of hepatocytes, in press, available on the
web, Biotechnology Progress
Leclerc E., Sakai Y., Fujii T., Perfusion Culture of Fetal Human
Hepatocytes in Microfluidic-Environments, in press, available
on the web, Biochemical Engineering Journal
Leclerc E., Furukawa K.S., Miyata F., Sakai
Y.,Ushida T., Fujii T., Fabrication of
microstructures in photosensitive biodegradable polymers for tissue engineering
applications, Biomaterials, 25,
4683-4690, 2004
Leclerc E.,
Furukawa K.S., Miyata F., Sakai Y., Ushida T., Fujii T.,
Effect on liver cells of stepwise microstructures fabricated in a
photosensitive biodegradable polymer by softlithography, Materials
Science and Engineering: C, 24:3, 349-354, 2004
Lattest conferences:
Leclerc
E., Sakai Y. and Fujii T., A Multi-Layer PDMS
Microfluidic Device for Tissue Engineering Applications, Proc. of MEMS-2003,
Kyoto, Japan, 2003
Leclerc
E, Sakai Y, Fujii T, Fetal Human Hepatocytes and
Endothelial Cells co-culture in a microfluidic environment, Proc of mTAS-2003, Tahoe Lake,
USA, 2003
Leclerc E.,
Furukawa K. S., Miyata, F., Sakai, Y., and Fujii, T., A Bioreactor for
Continuous Perfusion Cell Culture Fabricated with Photosensitive Biodegradable
Polymer, Proc. of Transducers-2003, Boston, USA, 2003
2000
Research Project (36 months):
“Study and
simulation of the growing and propagation phases of a steam explosion in the
framework of severe
accidents occurring in pressurized water nuclear reactor”
Abstract:
In hypothetical
Severe Accident studies for a PWR, a large amount of molten corium may be
poured into water. There is then a risk of Steam Explosion. After the premixing
sequence in which the melt is more or less dispersed into water, a fine
fragmentation process may start which can lead to an escalation. Such an event
is generally triggered by the destabilization of the vapor film surrounding the
hot melt droplets. In this work, an attempt to describe all the successive
processes leading to this fine fragmentation is presented. First a critical
analysis of previous models is performed, allowing us to propose a new sequence
of events. As in the previous models, the film destabilization leads to the
growth of cold liquids peaks induced by Rayleigh Taylor instability. As these
peaks have a smaller density than the drop, they do not penetrate into the hot
drop. At the cold liquid-hot liquid contacts, transient heat transfer leads to
the explosive boiling of a small amount of coolant. The generated local
pressurization deforms the hot melt interface. This can produce fine fragments
from the filaments issued from the melt. Some of them may reach the
vapor-coolant interface where intense and rapid vaporization occurs. A large
bubble then develops and a new fragmentation sequence may again appear at the
bubble collapse. The present model is supported by experimental results.
Keywords: Intense vaporization, thermal fragmentation, steam
explosion
Contact :
eric.leclerc@utc.fr
Contact : georges.berthoud@cea.fr
Linked publication:
Leclerc E, Berthoud G, Modeling of melt droplet
fragmentation following vapor film destabilisation by a trigger pulse, Nuclear
Technology, 144:2, 158-174, 2003
1997 Master thesis
keeping terms (4 months):
“Experimental and analytic
study of the influence of downstream flow conditions on Mach stem
height”, Laboratoire
du CNRS d’Aérothermique de Meudon .
Abstract:
An
experimental work on the influence of downstream flow expansion process on Mach
stem height has been carried out in steady hypersonic flows. The results showed
clearly for the first time that the Mach stem height does not depend on the
extent of expansion fan. These results contradict recent analytical findings
from which a strong influence of downstream flow conditions on Mach stem height
may be expected.
Keywords: Mach reflection, Mach stem, Expansion fan, Supersonic flow
Contact : eric.leclerc@utc.fr
Contact: A.Chpoun@iut.univ-evry.fr
Website: http://web.cnrs-orleans.fr/~webaero/index.html
Linked publication:
Chpoun A, Leclerc E, Experimental investigation
of the influence of downstream flow conditions on Mach Stem height, Shock
Waves, 9 : 269-271, 1999
1996 Undergraduate project (4
months):
« Influence of
hoar-frost on the helicopter rotor screw »
Laboratory of the University of Aix
Marseille II, with a mentor of Eurocopter INC (http://www.eurocopter.com/)